Database (2009) Vol. 2009:bap004;
doi:10.1093/database/bap004
published on
April 28, 2009
Published by Oxford University Press 2009.
This is Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The Homeodomain Resource: a comprehensive collection of sequence, structure, interaction, genomic and functional information on the homeodomain protein family
R. Travis Moreland
,
Joseph F. Ryan,
Christopher Pan and
Andreas D. Baxevanis*
Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA
*Corresponding author: Tel: +1 301 496 8570; Fax: +1 301 402 6858; Email: andy{at}nhgri.nih.gov
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Abstract
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The Homeodomain Resource is a curated collection of sequence,
structure, interaction, genomic and functional information on
the homeodomain family. The current version builds upon previous
versions by the addition of new, complete sets of homeodomain
sequences from fully sequenced genomes, the expansion of existing
curated homeodomain information and the improvement of data
accessibility through better search tools and more complete
data integration. This release contains 1534 full-length homeodomain-containing
sequences, 93 experimentally derived homeodomain structures,
101 homeodomain protein–protein interactions, 107 homeodomain
DNA-binding sites and 206 homeodomain proteins implicated in
human genetic disorders.
Database URL: The Homeodomain Resource is freely available and can be accessed at http://research.nhgri.nih.gov/homeodomain/
Received November 7, 2008; Accepted March 14, 2009
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Introduction
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Homeodomain-containing proteins are transcription factors that
play a critical role in various cellular processes, including
body plan specification, pattern formation and cell fate determination
during metazoan development
1. Members of this family are characterized
by a helix-turn-helix DNA binding motif known as the homeodomain.
X-ray crystallographic and NMR spectroscopic studies on several
homeodomain-containing proteins (
2–6) show that this motif
is comprised of three
-helices that are folded into a compact
globular structure with an N-terminal extension. Helices I and
II lie parallel to each other and across from the third helix.
This third helix is also referred to as the ‘recognition
helix’, as it confers DNA-binding specificity on individual
homeodomain proteins. Homeodomain-containing proteins may interact
with each other to enhance or mediate transcriptional activity,
either by the binding of multiple proteins to the same segment
of DNA or through the formation of DNA-independent complexes.
Nucleotide- and protein-level mutations associated with homeodomain
proteins can lead to a number of congenital abnormalities [c.f.
(
7,
8)]. The homeodomain structural motif is highly conserved
across eukaryotic species, and the expansion and diversification
of this family of proteins in various lineages has been shown
to coincide with the advent of major morphological innovations
(
9–12).
In recent years, studies utilizing high-throughput techniques have generated an extraordinary amount of information about these homeodomain proteins, but this information is not always easily accessible to the working biologist. For instance, recent large-scale genome sequencing efforts have led to the availability of complete collections of homeodomain proteins from an evolutionarily diverse set of species, but retrieving complete sets of homeodomain sequences from a particular species is not trivial. Likewise, while several large-scale projects aimed at computationally predicting protein–protein interactions through text mining and other similar approaches have been largely successful in terms of identifying potential relationships between proteins, identifying interactions specific to homeodomains remains an arduous task. In addition, the determination of 3D structures, identification of protein binding sites and our knowledge regarding the role of specific homeodomain proteins in disease causation has been steady, so keeping abreast of these discoveries remains challenging.
The Homeodomain Resource uses a combination of automated and manually verified extraction methods to yield a comprehensive collection of sequence, structure, interaction, genomic and functional information on the homeodomain family (13,14). In addition to a complete collection of homeodomains for 24 species (Table 1), the Homeodomain Resource contains information on DNA-binding targets, protein–protein interactions, 3D structures and homeodomains implicated in human disorders. Each annotation is manually curated, mapped to a specific protein and organism and fully cross-referenced to various external databases, including its primary citation in PubMed. Data are presented in an intuitive, user-friendly format and is keyword-searchable across all tables. Each reference in this database is rigorously selected to assure non-redundancy, and updates are performed on a continuous basis.
Examples of how data from the Homeodomain Resource have been
used in various biological contexts to date include studies
on the prediction of specific DNA-binding sites for homeodomain
proteins (
15), the analysis of non-conserved co-evolving positions
within functional sites in a variety of protein families (
16)
and the interpretation of phage display selection experiments
aimed at identifying elements within the engrailed homeodomain
responsible for sequence-specific DNA binding (
17). These data
have also been used to help interpret features found within
the structures of the stem cell transcription factor Nanog (
18) and the
Drosophila Bicoid–DNA complex (
19). Finally,
information from the Homeodomain Resource has been used as a
reference to aid in understanding mutation data from patients
with disorders such as idiopathic short stature and Leri-Weill
dyschondrosteosis (
20) and brachydactyly types D and E (
21).
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Database description
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The Homeodomain Resource has expanded significantly since its
last release [
Tables 1 and
2; (
13)], and substantial enhancements
have been made to the user interface to allow for easier navigation
and overall usability. Unlike previous versions of the database,
the current version connects all annotations in a relational
framework (
Figure 1), providing an integrated view of all the
analyses associated with a particular homeodomain protein. This
new system allows for a more powerful query engine that enables
a user to query across multiple annotations in a single search
(
Figure 2). Homeodomain Resource accession numbers are assigned
to each entry in the database to facilitate data sharing amongst
the user community. These accession numbers take the format
HDR
xn, where
x indicates the data category for the entry (e.g.
s = structures) and
n is a three-digit number identifying the
entry. In addition, the database is more genome-centric, with
an eye towards evolutionary studies. Whereas previous versions
relied heavily on choosing proteins that had annotations from
Swiss-Prot associated with them, this new edition places more
emphasis on compiling complete sets of homeodomains from a diverse
range of species. The combination of additional sequences, more
comprehensive datasets, and greater data connectivity provides
a much more powerful and robust resource to biologists.
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Figure 1. HDR relational framework. The relational design connecting the Homeodomain Resource's 14 primary tables are illustrated in the figure. Primary keys are indicated in red, foreign keys in green and keys characterized as both primary and foreign in blue. The new database design is centered around data found in the ‘Proteins’ table. All proteins are lineage-specific and linked to the ‘Organisms’ table. A single protein may contain one or more homeodomains related to the ‘Proteins_Homeodomains’ table. DNA-binding targets, protein–protein interactions, 3D structures and homeodomains implicated in human disorders are normalized and linked to the ‘Proteins’ table. External annotation from multiple databases are integrated via the ‘External_Ids’ table. Database entries are referenced with their primary citation via the ‘Publications’ table.
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Figure 2. The Homeodomain Resource provides a simple search query interface, allowing the user to either query part or all of the Resource (top). Selecting ‘Entire Database’ from the pull-down menu returns a summary screen, indicating how many entries of each type were identified (bottom). Clicking on any of the hyperlinked numbers in the table takes the user directly to that set of results. In addition, overall navigation within the site has been improved with the addition of sidebar tools and links to complete datasets in each homeodomain category.
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Homeodomain protein sequence entries
The sequence dataset in the Homeodomain Resource was assembled
by first utilizing data from a series of homeodomain surveys
of metazoan genomes (
22–24). Next, a hidden Markov model
(HMM) was generated from these aligned sequences using the HMMer
Toolkit (
25), and the HMM was subsequently used to search RefSeq
(
26) to identify additional members of the homeodomain family.
Alignments produced by HMMsearch (
25) were parsed using Perl
scripts; this was followed by manual alignment to the HMMsearch
alignment using GeneDoc (
27). Inspection (and manual adjustment)
of the alignments become necessary if HMMsearch introduces gaps
in biologically implausible locations within the sequence. One
such example involves the sequence of HDRp1895, which is truncated
at its N-terminus. HMMsearch introduced a gap of length 7 between
the next-to-last (R52) and the last (Q53) residue in the sequence;
in this case, the gap was removed, placing R52 directly next
to Q53, thereby producing a better-quality alignment. These
alignments are then added, along with annotations from Entrez
Gene (
28), to the Homeodomain Resource. The International Protein
Index (
29) was used to match Entrez Gene identifiers with entries
from other external resources, such as the Mouse Genome Database
(MGD;
30) and the Zebrafish Information Network (ZFIN;
31),
where possible. As of December 2008, 24 fully sequenced genomes
have been sampled: 8 metazoan (4 vertebrate and 4 invertebrate),
11 fungi, 4 protozoan and 1 plant (
Table 1). This process yielded
1534 protein entries. Individual protein entries are hyperlinked
to a detailed view that presents gene- and protein-level annotation,
full-lineage taxonomy and both the full-length and homeodomain-only
sequences. Annotations that refer to external resources are
hyperlinked to their source database (e.g. Entrez Gene).
The complete set of homeodomain proteins can be downloaded in FASTA format as either full-length sequences or homeodomain alignments. Alternatively, a customized dataset can be built either by selecting sequences resulting from a query or by manually selecting sequences from the entire dataset. Query results can be sorted to facilitate the construction of custom datasets. The ability to retrieve a complete set of aligned homeodomains from a range of species makes the Homeodomain Resource an invaluable first step in a phylogenetic analysis. For example, a researcher wanting to know the phylogenetic affinity of a previously undescribed homeodomain from a fungus could download an aligned dataset of homeodomains from several fungal species, align the undescribed homeodomain to this dataset and then run one or more phylogenetic algorithms on this alignment. Users interested in an evolution-based classification of homeodomain-containing proteins are also encouraged to explore HomeoDB (32), a complementary database focusing on homeobox gene phylogenetic classification.
Structures of homeodomain proteins and protein–DNA complexes
The homeodomain structures are manually compiled from the NCBI Entrez Structure database (33) and the Protein Data Bank (PDB; 34). Each structural entry is manually inspected to ensure that the solved structure contains the homeodomain region of the protein. Also noted is the experimental technique used to determine its structure (either X-ray diffraction or NMR spectroscopy). Information on solved 3D structures of both homeodomain proteins and protein–DNA complexes is available in a concise, columnar format. Protein name, PDB and MMDB accessions and the source organism are given for each entry, and the table can be sorted, as needed. For each entry, a link is also provided to a detailed view of that structure record, providing additional information such as experimental technique, PDB title and its primary PubMed reference. From this detailed view, users can follow links to the source Entrez Structure and PDB records, where one can view still images of the structure and download the 3D coordinates of a structure of interest. The detailed view also provides a link to the protein annotation within the Homeodomain Resource itself, as well as to the PubMed abstract corresponding to the primary literature citation listed in PDB.
Protein–protein interactions involving homeodomain proteins
The Homeodomain Resource contains a systematically and thoroughly curated catalogue of experimentally determined protein–protein interaction data for the homeodomain protein family. To the best of our knowledge, this collection represents the most comprehensive collection of protein–protein interaction annotations specific to the homeodomain family. Interaction data were collected through manual literature searches; essential information about the nature of the specific protein–protein interactions was then extracted from the experimental data presented in these manuscripts. The identification of articles containing relevant biological information from PubMed required the use of discriminatory MeSH terms, from specific to more general keyword search combinations. PubMed titles, abstracts and full text were searched for keywords that would be indicative of relevant protein–protein interactions (e.g. ‘DNA-independent interaction’). Interacting proteins were annotated and cross-linked to their corresponding protein entry within the Homeodomain Resource.
Protein–protein interaction data can be searched by publication information, interaction description and keyword data associated with their corresponding protein entries. Interaction data are returned in columnar format, listing the interacting proteins, the primary citation from the literature, the corresponding Biomolecular Interaction Network Database (BIND; 35) identifier and a link to a detailed view of the interaction. The detailed view provides additional information describing the homeodomain protein interacting regions, interacting residue locations and a description of the mechanism of interaction derived from the primary publication, as well as internal links to details on each of the interacting proteins within the HDR.
A new feature of this release is the cross-referencing of homeodomain protein–protein interaction data to their respective BIND interaction entries. BIND was queried for previously unreported homeodomain protein–protein interactions in parallel with the aforementioned PubMed literature searches, using general (e.g. ‘homeobox OR homeodomain AND interaction’) to more specific (e.g. ‘homeobox OR homeodomain AND interaction_object_type = protein AND NOT = DNA’) search criteria. Following a manual extraction of false positives, interactions from BIND were extracted and deposited in the Homeodomain Resource. All protein–protein interaction data derived from manual curation of PubMed have also been deposited into the BIND database. Each interaction derived from the Homeodomain Resource has been assigned a unique BIND accession number and is hyperlinked from BIND back to the Homeodomain Resource (Figure 3).
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Figure 3. Search results from a query of protein–protein interactions data for the interaction of homeodomain proteins Lhx2 and Msx1 (SEARCH ‘Protein-Protein Interactions’ FOR ‘MSX1’) (top). Each protein–protein interaction entry within the Homeodomain Resource is hyperlinked to the corresponding entry in BIND, which provides additional details on the mechanism(s) of interaction (bottom). See text for additional details.
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Homeodomain DNA-binding sites
DNA binding sites for homeodomain proteins have been obtained
through extensive review of the published literature, citations
in Online Mendelian Inheritance in Man (OMIM;
36,
37) and entries
for DNA-bound homeodomain structures from PDB. As with the interaction
data described above, binding site data can be searched by publication
information and by keyword data associated with its corresponding
protein entry. Binding site data are returned in columnar format;
the columns include homeodomain names, their respective DNA-binding
sequences and references to the primary citation from which
the information was retrieved. The core regions of each of the
DNA binding sites are shown in bold type. A detailed view of
the binding site record displays the consensus DNA sequence,
the corresponding PubMed reference and a link to details about
the protein; the protein details includes the Protein HDR identifier,
the common name of the protein, the gene symbol listed in Entrez
Gene and the UniProt protein accession.
Human genetic and genomic disorders linked to homeodomain proteins
Information on human genetic and genomic disorders linked to homeodomain proteins has been compiled from manual searches of both OMIM and the Human Gene Mutation Database (HGMD; 38). Any false positives resulting from the OMIM and HGMD searches were manually removed from the dataset. Manually derived entries from the previous Homeodomain Resource release were automatically compared and updated, while new automated entries were manually verified.
Each entry in the Disorders and Mutations dataset represents a single homeobox gene associated with one or more disease(s) or disorder(s). For each, the corresponding OMIM nucleotide- (e.g. 1-BP DEL, 504T) and/or protein-level (e.g. GLN140TER) mutations are shown. This dataset can be queried using any of the aforementioned fields, and the results can be sorted by clicking on the appropriate column field heading. Gene symbols are hyperlinked to the corresponding entry in the proteins table as well as to entries in HGMD (registration required).
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Technical improvements
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In addition to an overhaul of the interface, a number of back-end
technical modifications have been made to improve data collection,
storage and automation. A number of new Perl scripts have been
developed for this release which facilitate the automation and
updating of external annotation sources linked to the database,
thereby eliminating a number of manual steps previously required
for these processes. For example, a new set of Perl scripts
uses a list of existing gene symbols obtained from Swiss-Prot
to automatically search Entrez Gene, pairing protein-centric
annotation of existing homeodomain entries with their gene-centric
equivalent. A second set of Perl scripts parses Entrez data
via E-utilities, mapping a homeodomain entry to its corresponding
Disease and Disorders entry at OMIM. Each of the new entries
is examined manually and either added to the database or designated
as false positive. The search and update functions are executed
quarterly to update the disorders and mutations annotation.
Another Perl script was developed to parse the output of HMMsearch,
retrieve sequence and annotation information from Entrez, and
insert unique hits into the Homeodomain Resource. This approach
results in a relatively simple pipeline for adding new sequence
entries, thereby keeping this database current.
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Future considerations
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With these new tools in hand for importing complete sets of
homeodomain sequences from fully sequenced genomes, we intend
to continue to add sequence data from already-sequenced species.
We also intend to include additional homeodomain sequence data
from newly sequenced genomes, fully anticipating a new wave
of such data becoming available with the advent of new, next-generation
sequencing technologies.
It is becoming increasingly evident that homeodomain transcription factors have played and continue to play key roles in the evolution of eukaryotic species. Likewise, research in this area continually shows that disruptions in the wild-type function of this class of proteins underlie a significant number of devastating human disorders, as evidenced by the extensive list of genetic and genomic disorders catalogued in the Disorders and Mutations section of the Homeodomain Resource Web site. As a result, the amount of homeodomain-related data being generated—and the need for biologists to be able to process and consider these data—will be critical to the advancement of our understanding of these proteins. It is our intention to continue to maintain and update the Homeodomain Resource in the future, so as to provide a solid discovery framework for biologists and clinicians studying this important class of proteins.
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Funding
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This research was supported by the Intramural Research Program
of the National Human Genome Research Institute, National Institutes
of Health.
Conflict of interest. None declared.
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Notes
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The authors wish it to be known that, in their opinion, the
first two authors should be regarded as joint First Authors.
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Abstract
Introduction